Health
Fragile balance in the gut

Fragile balance in the gut

The presence of probiotics such as lactic acid bacteria changes the environment in the intestine and forces the yeast fungus Candida albicans to change its metabolism, making it less infectious. This way, probiotics can contain or prevent the spread of fungal infections in the gut. Researchers at the Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute (Leibniz-HKI) in Jena, Germany, have also found that intestinal cells actively promote bacterial growth to protect themselves from the fungus. The findings were published in Nature Communications.

The yeast Candida albicans naturally colonizes the human body, especially common in the intestine. Usually this is a benign colonization, as the immune system and a healthy gut microbiome keep it in check. However, if the microbiome gets out of balance or the immune system is severely compromised, C. albicans can enter the bloodstream. This can be life-threatening especially for immunocompromised people in intensive care units.

Researchers at Leibniz-HKI have now found that human intestinal cells play an important role in fighting fungal infections caused by C. albicans. “The intestinal cells nourish lactic acid bacteria, which thereby multiply and in turn take nutrients away from the yeast fungus,” explains first author Raquel Alonso-Roman. The new conditions force C. albicans to adapt its metabolism, causing it to shed certain characteristics and  making it less infectious. Adding the probiotics to the gut creates a balance between yeast, lactic acid bacteria and the rest of the microbiome, which restores a healthy state.

Infections with C. albicans, such as vulvovaginal infections, are already successfully treated with lactic acid bacteria. “We already know that lactic acid bacteria in particular can counteract a fungal infection, prevent it or even kill the fungus. Our work now addresses the question of ‘how’,” explains Bernhard Hube, head of the Department of Microbial Pathogenicity Mechanisms.

In collaboration with systems biologists at the institute, the researchers developed computer models that can predict how lactic acid bacteria of the species Lactobacillus rhamnosus behave when encountering C. albicans. “Using data from previous studies, our computer models can predict that the lactic acid bacteria would multiply and eventually counteract C. albicans,” explains Mark Gresnigt, junior group leader at Leibniz-HKI. However, subsequent experiments in the lab showed that the bacteria did not multiply in conventional culture media as predicted. “Only when we added epithelial cells from the gut did the lactic acid bacteria begin to multiply,” Gresnigt continued. Together with the junior research group of Slavena Vylkova, also at Leibniz-HKI, the researchers were able to find out how the yeast fungus changes its metabolism to adapt to the new conditions. Since there are no longer enough nutrients in the intestine, there is an adjustment in the gene activity of C. albicans, which makes the yeast fungus less infectious and thus no longer able to damage the intestinal cells.

The results of this research form an important step forward in the fight against life-threatening fungal infections. “We want to find out how probiotics fight infection. With this knowledge, we may be able to develop measures to prevent the aggressive behavior of the fungus. The idea is to influence the fungus the way probiotics would, without actually using them,” Hube explains. Especially in immunosuppressed patients, he said, it is usually too dangerous to use live bacteria as a remedy.

The balance and dysbalance of microorganisms in humans, animals and the environment are the central research focus of the Balance of the Microverse Cluster of Excellence and the Collaborative Research Center FungiNet, which supported this work.

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